Diabetes mellitus is a chronic degenerative disease that causes long-term complications\nand represents a serious public health problem. In this manuscript, acankoreagenin isolated from\nthe leaves of Acanthopanax gracilistylus (LAG) is thought to possess excellent anti-diabetic properties.\nIn vitro, anti-diabetic activities were assessed based on the inhibitory activities with �±-glucosidase\n(IC50 13.01 �¼M), �±-amylase (IC50 30.81 �¼M), and PTP1B (IC50 16.39 �¼M). Acankoreagenin showed\nbetter anti-diabetic effects. Then, an investigation was performed to analyze the insulin secretion\neffects of the insulin-secreting cell line in RIN-m5F cells. It was found that acankoreagenin could\nincrease the insulin release in RIN-m5F cells. It was also found that acankoreagenin reduced NO\nproduction, activity of caspase-3, and the reactive oxygen species levels in the cells injured by\nprocessing of cytokines. In western blotting, inactivation of NF-�ºB signaling was confirmed.\nAcankoreagenin (20 �¼M) showed a higher I-�ºB�± expression and lower NF-�ºB expression than the\ncontrol group and showed a better expression than the positive control L-NAME (1 mM) (p < 0.05).\nThis study demonstrates the anti-diabetic effects of acankoreagenin in vitro and suggests\nacankoreagenin might offer therapeutic potential for treating diabetes mellitus.
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